Journal: The Journal of Physiological Sciences : JPS

Article Title: Senescent endothelial progenitor cells from dogs with pulmonary arterial hypertension: a before–after self-controlled study

doi: 10.1007/s12576-009-0053-7

Figure Lengend Snippet: CD133/KDR-positive cells were detected in peripheral blood and identified as circulating EPCs by flow cytometry using counting beads tubes. a Gated and counted beads from a fluorescein isothiocyanate (FITC) versus side-scattering corner (SSC) dot plot. Quadrants were set on the basis of isotype-identical controls. b Dual-positive CD133/KDR cells were identified and numerated in the upper right quadrant of the scatter plot from blood of dogs. The representative dot plots are represented. The absolute counts of positive cells per ml blood were calculated using the equation described in “Methods”. c Comparison of circulating EPCs number before and after PAH. d Differentiated EPCs were significantly reduced in PAH but transiently raised at the early stage after DHMC. Data are mean ± SD. *p < 0.05 versus baseline, # p < 0.05 versus 48 h

Article Snippet: Briefly, 50 μl of well-mixed anticoagulated whole blood was incubated with PE-labeled mouse anti-dog KDR antibody (Becton Dickinson) and APC-labeled mouse anti-human CD133 antibody (Miltenyi Biotec, Krohne, Germany) in manufacturer-recommended concentrations just above the stainless steel retainer of the tube for 30 min at 4°C.

Techniques: Flow Cytometry, Comparison